AVIAN INFLUENZA Antibody Test Kit

Avian Influenza Antibody 

The AI ELISA kit will measure the amount of antibody to AI in the serum of chickens and Turkeys. Microtitre plates have been pre-coated with inactivated AI antigen. Serum samples are diluted and added to the microtitre wells where any anti-AI antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti-AI antibodies originally bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti-AI antibody is present and the intensity is directly related to the amount of anti-AI present in the sample.

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