Mhyo
The Mycoplasma hyopneumoniae antibody ELISA kit will measure the amount of antibody to M.hyopneumoniae in the serum of pigs. Microtitre plates have been pre-coated with inactivated Mhyo. Samples taken from pigs are diluted and added to the microtitre wells where any anti-Mycoplasma hyopneumoniae antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other sample proteins are then washed away. Anti-pig antibody labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any Swine anti-Mhyo originally bound to antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if any anti-Mhyo antibody is present and the intensity is directly related to the amount of antibody present in the sample.
