Newcastle Disease Antibody Test Kit
The NDV ELISA kit will measure the amount of antibody to NDV in the serum of chickens. Microtitre plates have been pre-coated with inactivated NDV antigen. Chicken serum samples are diluted and added to the microtitre wells where any anti- NDV antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti- NDV antibodies originally bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti- NDV antibody is present and the intensity is directly related to the amount of anti- NDV present in the sample.
Additional Information
Product type: | Poultry |
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Product code: | CK116 NDV |
Classification: | Antibody test |
Technique: | Indirect ELISA |
Intended use: | To detect antibodies to Newcastle disease virus in chickens and turkeys |
Kit format: | Plates per kit: 5 Maximum sample size: 460 Run at room temperature Incubation times: 30-30-15 Read at 405 NM 1:500 dilution |
Data pack available: | Upon request |
Reference controls: | Reference Control 11 available (see accessories list) |
